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The qPCR primers have a little bit lessen but sufficiently equivalent protection to the domain Microbes in comparison to the primers for NGS. The protection of both equally qPCR and NGS primer sets is very similar for that four taxa picked for taxon-distinct qPCR (S1B Desk), which provides reliable validation in the described approach in this examine. For long term improvement on the herein introduced method, a qPCR assay utilizing the exact same primer pair as for NGS may very well be optimized and validated.

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